Novel Coating for Microarrays & Biosensors

Universal coating, high thermal stability, high sensitivity, low backgrounds

1.    Characterization of porous alumina membranes for efficient, real-time, flow through biosensing. Laura Sola, Jesús Álvarez, Marina Cretich, Marcus J. Swann, Marcella Chiari, Daniel Hill. Journal of Membrane Science, Volume 476, 15 February 2015, Pages 128-135.

2.    Real time optical immunosensing with flow-through porous alumina membranes. Jesús Álvarez, Laura Sola, Marina Cretich, Marcus J. Swann, Kristinn B. Gylfason, Tormod Volden, Marcella Chiari, Daniel Hill. Sensors and Actuators B: Chemical, Volume 202, 31 October 2014, Pages 834-839.

3.    Multi-spot, Label-free Detection of Biomarkers in Complex Media by Reflectionless Surfaces. M. Salina, F. Giavazzi, E. Ceccarello, F. Damin, M. Chiari, M. Ciuffo, G.P. Accotto, M. Buscaglia. Procedia Engineering, Volume 87, 2014, Pages 58-61.

4.    A fast and simple label-free immunoassay based on a smartphone. Fabio Giavazzi, Matteo Salina, Erica Ceccarello, Andrea Ilacqua, Francesco Damin, Laura Sola, Marcella Chiari, Bice Chini, Roberto Cerbino, Tommaso Bellini, Marco Buscaglia. Biosensors and Bioelectronics, Volume 58, 15 August 2014, Pages 395-402.

5.    Characterization of a new fluorescence-enhancing substrate for microarrays with femtomolar sensitivity. Marina Cretich, Clelia Galati, Lucio Renna, Guglielmo Guido Condorelli, Paola Gagni, Marcella Chiari. Sensors and Actuators B: Chemical, Volume 192, 1 March 2014, Pages 15-22.

6.    Photolithographic bio-patterning of magnetic sensors for biomolecular recognition. E. Albisetti, D. Petti, F. Damin, M. Cretich, A. Torti, M. Chiari, R. Bertacco. Sensors and Actuators B: Chemical, Volume 200, September 2014, Pages 39-46.

7.    Functionalization of gold surfaces with copoly(DMA-NAS-MAPS) by dip coating: Surface characterization and hybridization tests. D. Petti, A. Torti, F. Damin, L. Sola, M. Rusnati, E. Albisetti, A. Bugatti, R. Bertacco, M. Chiari. Sensors and Actuators B: Chemical, Volume 190, January 2014, Pages 234-242

8.    Nanoscale characterization of DNA conformation using dual-color fluorescence axial localization and label-free biosensing. Zhang X, Daaboul GG, Spuhler PS, Freedman DS, Yurt A, Ahn S, Avci O, Ünlü MS. Analyst. 2014 Dec 21;139(24):6440-9. doi: 10.1039/c4an01425a.

9.    Digital sensing and sizing of vesicular stomatitis virus pseudotypes in complex media: a model for ebola and marburg detection. Daaboul GG, Lopez CA, Chinnala J, Goldberg BB, Connor JH, Unlü MS. ACS Nano. 2014 Jun 24;8(6):6047-55. doi: 10.1021/nn501312q. Epub 2014 Jun 4.

10.     A high-throughput method to examine protein-nucleotide interactions identifies targets of the bacterial transcriptional regulatory protein fur. Yu C, Lopez CA, Hu H, Xia Y, Freedman DS, Reddington AP, Daaboul GG, Unlü MS, Genco CA. PLoS One. 2014 May 8;9(5):e96832. doi: 10.1371/journal.pone.0096832. eCollection 2014.

11.     LED-based interferometric reflectance imaging sensor for the detection of amyloid-β aggregation. Xin R. Cheng, George G. Daaboul, M. Selim Ünlü and Kagan Kerman. Analyst, 2014,139, 59-65.

12.     One-pot phase transfer and surface modification of CdSe-ZnS quantum dots using a synthetic functional copolymer. Finetti C, Colombo M, Prosperi D, Alessio G, Morasso C, Sola L, Chiari M. Chem Commun (Camb). 2014 Jan 7;50(2):240-2. doi: 10.1039/c3cc46086j.

13.     Universal hydrophilic coating of thermoplastic polymers currently used in microfluidics. Zilio C, Sola L, Damin F, Faggioni L, Chiari M. Biomed Microdevices. 2014 Feb;16(1):107-14. doi: 10.1007/s10544-013-9810-8. Erratum in: Biomed Microdevices. 2014 Jun;16(3):499.

14.     Allergen immobilisation and signal amplification by quantum dots for use in a biosensor assay of IgE in serum. Platt GW, Damin F, Swann MJ, Metton I, Skorski G, Cretich M, Chiari M. Biosens Bioelectron. 2014 Feb 15;52:82-8. doi: 10.1016/j.bios.2013.08.019. Epub 2013 Aug 27.

15.     An interferometric reflectance imaging sensor for point of care viral diagnostics. Reddington AP, Trueb JT, Freedman DS, Tuysuzoglu A, Daaboul GG, Lopez CA, Karl WC, Connor JH, Fawcett H, Ünlu MS. IEEE Trans Biomed Eng. 2013 Dec;60(12):3276-83. doi: 10.1109/TBME.2013.2272666.

16.     High-throughput label-free detection of DNA hybridization and mismatch discrimination using interferometric reflectance imaging sensor. Ahn S, Freedman DS, Zhang X, Unlü MS. Methods Mol Biol. 2013;1039:181-200. doi: 10.1007/978-1-62703-535-4_16.

17.     A mass-tagging approach for enhanced sensitivity of dynamic cytokine detection using a label-free biosensor. Ahn S, Freedman DS, Massari P, Cabodi M, Ünlü MS. Langmuir. 2013 Apr 30;29(17):5369-76. doi: 10.1021/la400982h. Epub 2013 Apr 18.

18.     Single nanoparticle detection for multiplexed protein diagnostics with attomolar sensitivity in serum and unprocessed whole blood. Monroe MR, Daaboul GG, Tuysuzoglu A, Lopez CA, Little FF, Unlü MS. Anal Chem. 2013 Apr 2;85(7):3698-706. doi: 10.1021/ac4000514. Epub 2013 Mar 20.

19.     Surface modifications by polymers for biomolecule conjugation. Sola L, Cretich M, Damin F, Chiari M. Methods Mol Biol. 2013;1025:95-107. doi: 10.1007/978-1-62703-462-3_7.

20.     Development of a high-sensitivity immunoassay for amyloid-beta 1-42 using a silicon microarray platform. Gagni P, Sola L, Cretich M, Chiari M. Biosens Bioelectron. 2013 Sep 15;47:490-5. doi: 10.1016/j.bios.2013.03.077. Epub 2013 Apr 6.

21.     Development of a high-sensitivity immunoassay for amyloid-beta 1-42 using a silicon microarray platform. Gagni P, Sola L, Cretich M, Chiari M. Biosens Bioelectron. 2013 Sep 15;47:490-5. doi: 10.1016/j.bios.2013.03.077. Epub 2013 Apr 6.

22.     Conditions for efficient on-chip magnetic bead detection via magnetoresistive sensors. E. Albisetti, D. Petti, M. Cantoni, F. Damin, A. Torti, M. Chiari, R. Bertacco. Biosensors and Bioelectronics, Volume 47, 15 September 2013, Pages 213-217.

23.     Leveraging on nanomechanical sensors to single out active small ligands for β2-microglobulin. Giulio Oliviero, Marcella Chiari, Ersilia De Lorenzi, Raffaella Colombo, Marina Cretich, Francesco Damin, Stefania Federici, Laura E. Depero, Paolo Bergese. Sensors and Actuators B: Chemical, Volume 176, January 2013, Pages 1026-1031.

24.     Non-invasive prenatal diagnosis of genetic diseases by advanced technologies. S. Galbiati, A. Monguzzi, N. Soriani, S. Stenirri, F. Lalatta, M. Seia, G. Restagno, F. Damin, M. Chiari, M. Ferrari. Clinical Biochemistry, Volume 46, Issue 12, August 2013, Pages 1154-1155.

25.     Precisely controlled smart polymer scaffold for nanoscale manipulation of biomolecules. Spuhler PS, Sola L, Zhang X, Monroe MR, Greenspun JT, Chiari M, Ünlü MS. Anal Chem. 2012 Dec 18;84(24):10593-9. doi: 10.1021/ac3018263. Epub 2012 Dec 7.

26.     TATA binding proteins can recognize nontraditional DNA sequences. Ahn S, Huang CL, Ozkumur E, Zhang X, Chinnala J, Yalcin A, Bandyopadhyay S, Russek S, Unlü MS, DeLisi C, Irani RJ. Biophys J. 2012 Oct 3;103(7):1510-7. doi: 10.1016/j.bpj.2012.08.030. Epub 2012 Oct 2.

27.     Interferometric silicon biochips for label and label-free DNA and protein microarrays. Cretich M, Monroe MR, Reddington A, Zhang X, Daaboul GG, Damin F, Sola L, Unlu MS, Chiari M. Proteomics. 2012 Oct;12(19-20):2963-77. doi: 10.1002/pmic.201200202. Epub 2012 Sep 24. Review.

28.     Quantification of surface etching by common buffers and implications on the accuracy of label-free biological assays. Ahn S, Spuhler PS, Chiari M, Cabodi M, Ünlü MS. Biosens Bioelectron. 2012 Jun-Jul;36(1):222-9. doi: 10.1016/j.bios.2012.04.020. Epub 2012 Apr 21.

29.     A nanoelectromechanical biosensor based on precise quantification and control of DNA orientation. Spuhler PS, Sola L, Zhang X, Monroe M, Greenspun J, Chiari M, Unlu MS. Conf Proc IEEE Eng Med Biol Soc. 2011;2011:4774-5. doi: 10.1109/IEMBS.2011.6091182.

30.     Multiplexed method to calibrate and quantitate fluorescence signal for allergen-specific IgE. Monroe MR, Reddington AP, Collins AD, LaBoda C, Cretich M, Chiari M, Little FF, Unlü MS. Anal Chem. 2011 Dec 15;83(24):9485-91. doi: 10.1021/ac202212k. Epub 2011 Nov 22.

31.     Overcoming mass transport limitations to achieve femtomolar detection limits on silicon protein microarrays. Cretich M, Bagnati M, Damin F, Sola L, Chiari M. Anal Biochem. 2011 Nov 1;418(1):164-6. doi: 10.1016/j.ab.2011.07.004. Epub 2011 Jul 14.

32.     Silicon biochips for dual label-free and fluorescence detection: Application to protein microarray development. Cretich M, Reddington A, Monroe M, Bagnati M, Damin F, Sola L, Unlu MS, Chiari M. Biosens Bioelectron. 2011 May 15;26(9):3938-43. doi: 10.1016/j.bios.2011.03.016. Epub 2011 Apr 9.

33.     Biomolecular detection employing the Interferometric Reflectance Imaging Sensor (IRIS). Lopez CA, Daaboul GG, Ahn S, Reddington AP, Monroe MR, Zhang X, Irani RJ, Yu C, Genco CA, Cretich M, Chiari M, Goldberg BB, Connor JH, Ünlü MS. Vis Exp. 2011 May 3;(51). pii: 2694. doi: 10.3791/2694.

34.     Label-free multiplexed virus detection using spectral reflectance imaging. Lopez CA, Daaboul GG, Vedula RS, Ozkumur E, Bergstein DA, Geisbert TW, Fawcett HE, Goldberg BB, Connor JH, Unlü MS. Biosens Bioelectron. 2011 Apr 15;26(8):3432-7. doi: 10.1016/j.bios.2011.01.019. Epub 2011 Jan 22.

35.     Synergetic chemiluminescence and label-free dual detection for developing a hepatitis protein array. C. Pereira, A. Yalçın, M. Cretich, M. Chiari, M.S. Ünlü, D. Nunes, D.A. Bergstein. Journal of Immunological Methods, Volume 371, Issues 1–2, 31 August 2011, Pages 159-164

36.     Label-free microarray imaging for direct detection of DNA hybridization and single-nucleotide mismatches. Ozkumur E, Ahn S, Yalçin A, Lopez CA, Cevik E, Irani RJ, DeLisi C, Chiari M, Unlü MS. Biosens Bioelectron. 2010 Mar 15;25(7):1789-95. doi: 10.1016/j.bios.2009.12.032. Epub 2010 Jan 4.

37.     Peptide microarrays on coated silicon slides for highly sensitive antibody detection. Cretich M, Damin F, Longhi R, Gotti C, Galati C, Renna L, Chiari M. Methods Mol Biol. 2010;669:147-60. doi: 10.1007/978-1-60761-845-4_12.

38.     Allergen microarrays on high-sensitivity silicon slides. Cretich M, Breda D, Damin F, Borghi M, Sola L, Unlu SM, Burastero SE, Chiari M. Anal Bioanal Chem. 2010 Oct;398(4):1723-33. doi: 10.1007/s00216-010-4077-x. Epub 2010 Aug 22.

39.     Coating of nitrocellulose for colorimetric DNA microarrays. Cretich M, Sedini V, Damin F, Pelliccia M, Sola L, Chiari M. Anal Biochem. 2010 Feb 1;397(1):84-8. doi: 10.1016/j.ab.2009.09.050. Epub 2009 Oct 2.

40.     Epitope mapping of human chromogranin A by peptide microarrays. Cretich M, Longhi R, Corti A, Damin F, Di Carlo G, Sedini V, Chiari M. Methods Mol Biol. 2009;570:221-32. doi: 10.1007/978-1-60327-394-7_10. Review.

41.     High sensitivity protein assays on microarray silicon slides. Cretich M, di Carlo G, Longhi R, Gotti C, Spinella N, Coffa S, Galati C, Renna L, Chiari M. Anal Chem. 2009 Jul 1;81(13):5197-203. doi: 10.1021/ac900658c.

42.     Direct observation of conformation of a polymeric coating with implications in microarray applications. Yalçin A, Damin F, Ozkumur E, di Carlo G, Goldberg BB, Chiari M, Unlü MS. Anal Chem. 2009 Jan 15;81(2):625-30. doi: 10.1021/ac801954x.

43.     A biofunctional polymeric coating for microcantilever molecular recognition. Oliviero G, Bergese P, Canavese G, Chiari M, Colombi P, Cretich M, Damin F, Fiorilli S, Marasso SL, Ricciardi C, Rivolo P, Depero LE. Anal Chim Acta. 2008 Dec 23;630(2):161-7. doi: 10.1016/j.aca.2008.09.069. Epub 2008 Oct 17.

44.     Protein microarrays enhanced performance using nanobeads arraying and polymer coating. Marquette CA, Cretich M, Blum LJ, Chiari M. Talanta. 2007 Feb 28;71(3):1312-8. doi: 10.1016/j.talanta.2006.06.039. Epub 2006 Aug 14.

45.     Surface behavior and molecular recognition in DNA microarrays from N,N-dimethylacrylamide terpolymers with activated esters as linking groups. Suriano R, Levi M, Pirri G, Damin F, Chiari M, Turri S. Macromol Biosci. 2006 Sep 15;6(9):719-29.

46.     A new polymeric coating for protein microarrays. Cretich M, Pirri G, Damin F, Solinas I, Chiari M. Anal Biochem. 2004 Sep 1;332(1):67-74.

47.    Characterization of a polymeric adsorbed coating for DNA microarray glass slides. Pirri G, Damin F, Chiari M, Bontempi E, Depero LE. Anal Chem. 2004 Mar 1;76(5):1352-8.

48.    Discrimination of molecular thin films by surface-sensitive time-resolved optical spectroscopy. Peli, S., Nembrini, N., Damin, F., Chiari, M., Giannetti, C., Banfi, F., Ferrini, G. APPLIED PHYSICS LETTERS, 2015, 107, 16.

49.    New "clickable" polymeric coating for glycan microarraysZilio, C., Bernardi, A., Palmioli, A., Salina, M., Tagliabue, G., Buscaglia, M., Consonni, R., Chiari, M. 2015, SENSORS AND ACTUATORS B-CHEMICAL, 2015, 412-420

50.    Digital detection of biomarkers assisted by nanoparticles: application to diagnostics. Cretich, M., Daaboul, GG., Sola, L., Unlu, MS., Chiari, M. TRENDS IN BIOTECHNOLOGY, 2015, 33, 343-351.

51.    DNA microarray-based solid-phase PCR on copoly (DMA-NAS-MAPS) silicon coated slides: An example of relevant clinical application.Damin, F., Galbiati, S., Ferrari, M., Chiari, M. BIOSENSORS & BIOELECTRONICS, 2016, 258, 3750.

52.    Reactive Microcontact Printing of DNA Probes on (DMA-NAS-MAPS) Copolymer-Coated Substrates for Efficient Hybridization Platforms. Castagna, R., Bertucci, A. , Prasetyanto, EA., Monticelli, M. , Conca, DV ., Massetti, M., Sharma, PP., Damin, F., Chiari, M.; De Cola, L.; Bertacco, R. LANGMUIR, 2016, 32, 3308-3313

53.    Multi-spot, label-free detection of viral infection in complex media by a non-reflecting surface. Salina, M., Giavazzi, F., Ceccarello, E., Damin, F., Chiari, M., Ciuffo, M., Accotto, GP., Buscaglia, M. SENSORS AND ACTUATORS B-CHEMICAL, 2016, 223, 957-962.

54.    Clickable Polymeric Coating for Oriented Peptide Immobilization. Sola, L., Gori, A., Cretich, M., Finetti, C., Zilio, C., Chiari, M. PEPTIDE MICROARRAYS: METHODS AND PROTOCOLS, 2ND EDITION, 2016, Book Series: Methods in Molecular Biology, Volume: 1352, Pages: 167-182, Editors: Cretich M, Chiari M. Cretich, M.

55.    Combined mass quantitation and phenotyping of intact extracellular vesicles by a microarray platform. Gagni, P., Cretich, M., Benussi, L., Tonoli, E., Ciani, M., Ghidoni, R., Santini, B., Galbiati, E. 2016, ANALYTICA CHIMICA ACTA, 902, 160-167.

56.    Multi-spot, label-free immunoassay on reflectionless glass. Salina, M., Giavazzi, F., Lanfranco, R., Ceccarello, E., Sola, L., Chiari, M., Chini, B., Cerbino, R., Bellini, T., Buscaglia, M. BIOSENSORS & BIOELECTRONICS, 2016, 74, 539-545.

57.    Novel polymeric coatings with tailored hydrophobicity to control spot size and morphology in DNA microarray, Sola, L Damin, F Cretich, M Chiari, M., SENSORS AND ACTUATORS B-CHEMICAL, 2016,  231, 412-422.

58.    Click Chemistry Immobilization of Antibodies on Polymer Coated Gold Nanoparticles, Finetti, C., Sola, L., Pezzullo, M., Prosperi, D., Colombo, M., Riva, B., Avvakumova, S.,  Morasso, C., Picciolini, S., Chiari, M., LANGMUIR, 2016, 32, 7435-7441.

59.    Synthesis of Clickable Coating Polymers by Postpolymerization Modification: Applications in Microarray Technology Sola, L., Damin, F., Gagni P., Consonni, R., Chiari, M.,  Langmuir, 2016, 32 (40), pp 10284–10295

60.    Digital detection of exosomes by interferometric imaging, Chiari, M., Daaboul, G., Gagni, P., Benussi, L., Bettotti, P., Ciani, M., Cretich, M., Freedman, D., Ghidoni, R., Yalcin Ozkumur, A., Piotto, C., Prosperi, D., Santini B., and Ünlü, M.S.,  Scientific Reports (2016) 6:  3746