Can I make the coating outside a chemistry laboratory?
Yes, the polymer is not cytotoxic and all the ingredients required for the coating are safe.
Can I decide the number of slides that I can coat?
Yes, the polymer comes as a 50X solution that could be diluted in aqueous buffer before applying the coating; one can prepare the solution required and store the remaining polymer for another coating section.
How stable is the polymer solution?
The polymer solution is stable for one year when stored at -20°C with desiccant.
How stable are the coated slides?
The slides can be stored for six months at room temperature in a sealed package with desiccant.
How much coating solution do I need for one slide?
We recommend 1 mL per slide but it depends on the application, in some cases only one side of the slide can be coated.
Do I need to pre-treat the surface before applying the coating
It depends on the material you want to coat and on its level of purity, silicon oxide, nitride and glass do not necessary need a pre-treatment, gold and plastics should be oxygen plasma treated before applying the coating.
Do I need to dry the slides extensively after the coating application?
Yes, we recommend to dry the slides in a vacuum oven at 80°C but if you don't have this equipment it is possible to seal the slides under vacuum and dry them in a regular oven.
Which MCP-polymer should I use for different applications?
We suggest MCP-4 for DNA and peptide microarrays, MCP-2 for protein microarrays, MCP-2F or MCP-4F when the distance between spots is lower than 130 ump or for hydrophobic plastics, MCP-click for oriented immobilization of peptides and DNA. In general MCP-click is not sensitive to hydrolysis it provides higher grafting density.
Our synthesis processes are based on techniques refined over many years in the academic labs of our founders, Annelise Barron and Marcella Chiari. Thousands of hours of research have gone into every product we manufacture. Unlike many uncontrolled bulk polymerization methods, we use emulsion polymerization, which allows for superior heat transfer to a system of essentially billions of microreactors delivering unprecedented control.
Every step of the process is well controlled and traceable, following ISO 9001:2008 quality standards. We test each product to ensure conformance to specifications using equipment that is calibrated using NIST-traceable standards.