Technologies

​Lucidant Polymers is equipped for multiple product syntheses using an array of chemical process equipment and characterization tools. Our production equipment is closely tracked and maintained under ISO 9001:2008 requirements. Our separate research and development laboratory space allows us to develop and fully characterize new processes for customers with an ability to manufacture a final product to ISO standards in our Sunnyvale, California facility.

Microarray Coatings 

Lucidant Coating Polymers

Lucidant offers a wide range of functional polymers that allow to coat slides of different materials for DNA and protein microarray. The coating procedure is fast, simple, and can be performed in any laboratory. Link to the procedure. The use of polymeric materials for modifying/functionalizing substrates is one of the most effective ways for tailoring surface characteristics

Characteristics of the surface coating:

Polymer selection

All MCP polymers form a coating on the following materials: Glass, silicon oxide, silicon nitride, gold, PDMS, COC, and Teflon.
 


Chemical structure for MCP-2 and MCP-4


Immobilization of oligonucleotides on silicon oxide surface: (left) label free, interferometric image of surface spots, (right) fluorescence intensity after hybridization with CY3 labeled complementary oligonucleotide.  
 


The lower surface energy of MCP-2F prevents spreading and merging of the spots


Spots on MCP-2F (pitch 160 microns) versus a standard spotting formulation at small pitch spacing of 160 microns on silicon substrate
 



Video demonstration of MCP application

Procedure for Coating Microarray Slides with MCP-2 (< 40 minute process)


Overview Lucidant coatings

We have prepared a summary of the scientific literature that cites the use of Lucidant microarray polymers. The publications have been categorized by the type of slide coating used, probes printed, and application. This is a useful guide when deciding which of the Lucidant polymers to use with any particular application. Link to the publication

Lucidant Coating Kits (MCP-2, -2F, -4, and others)

MCP polymer stability: 1 year when properly stored at -20°C.

Images of the chips coated with MCP polymers stored at -20°C (Laser power and PTM gain 55_55% Scanner Perkin Elmer)

Spot Fluorescence intensity on chips coated with MCP polymers stored at -20°C for 1 month, 6 months and 12 months.

Lucidant Polymers to modulate EOF in capillary electrophoresis

Capillary electrophoresis (CE) is a powerful analytical technique that combines the advantages of electrophoretic separation with automation, allowing for a fast and efficient separation of a wide variety of compounds, including chemical, pharmaceutical and biological samples.

To obtain highly efficient and reproducible CE separations of proteins, peptides and DNA an effective modification of the surface of the capillary is necessary to:

CE coatings must withstand shear forces and competition with various species present in the running buffer (urea, detergents and proteins). Therefore, methods that provide stable interfaces, even under harsh conditions, are generally preferred. Lucidant has introduced a family of polymers that allows to form a covalent coating on the capillary wall by a process that conjugates physi- and chemisorption.

Lucidant MCP-3 is a copolymer of N,N-dimethylacrylamide (DMA), N- and 3-(trimethoxysilyl) propyl methacrylate (MAPS) bearing epoxy groups. By a fully authomated protocol this polymer Link to the protocol, sold as powder, forms a stable coating in 3hours.  

Schematic representation of MCP-3 coating

MCP-3 Lucidant polymer coatings suppresses EOF to a negligile value    

Separation of alkaline proteins in MCP-3 coated capillary

Experimental conditions:

Capillary L=54 cm ID= 50

Coating online with MCP-3

EOF pH 8.5= 3 x 10-11 m2/Vsec

EOF pH 2.2 AcOH =  -2 x 10-10 m2/Vsec

Alkaline mix: lysozime, cytochrome C, ribonuclease A 1 mg/mL in DI water

Injection 0.3 psi 2 sec

Separation 30 kV

Running buffers:

Separation of alkaline proteins in MCP-3 coated capillaries

Polyacrylamides

Polyacrylamides, gels, and acrylamide monomers

Lucidant's core technical expertise is polyacrylamide chemistry for electrophororetic applications. Our specialized manfacturing process allows synthesis of polymers with targeted, precisely controlled molecular weights up to a massive 25MDa. Lucidant can synthesize linear or cross-linked polyacrylamides to customer specs. Material can be supplied as bulk powder or as a ready-to-use gel.  Order here

In addition, Lucidant can provide novel acrylamide monomers that enhance the stability, separation properties, or hydrophobicity of polyacrylamide gels. If you have an existing need or a novel application, please contact us with your requirements.

Click for information on Lucidant Allyl PVA

Click for information on Lucidant ELPA 1